thermal cycle dice real time system iii (TaKaRa)

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TaKaRa thermal cycle dice real time system iii
Thermal Cycle Dice Real Time System Iii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 8002 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermal+cycle+dice+real+time+system+iii/Thermal+Cycler+Dice+Real+Time+System/pm41666942-133-9-16
Average 98 stars, based on 8002 article reviews

thermal cycle dice real time system iii - by Bioz Stars, 2026-09

98/100 stars

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Article Title: Extracellular Vesicle Inhibitors Enhance Cholix-Induced Cell Death via Regulation of the JNK-Dependent Pathway
Article Snippet: .. To evaluate the mRNA expression levels, qPCR analysis was performed using the KOD SYBER qPCR Mix (TOYOBO, Tokyo, Japan) and the Thermal Cycle Dice Real-Time System III (Takara Bio). ..

Real-time Polymerase Chain Reaction:

Article Title: Lipid nanoparticles containing zwitterionic lipids versatilely enhance the efficiency of mRNA delivery.
Article Snippet: The extracted RNA (0.5 μg/6 μL) was reverse-transcribed using a complementary deoxyribonucleic acid (cDNA) synthesis kit (Toyobo, #FSK-301) according to the manufacturer's protocol. .. Then, qPCR was conducted with the use of a Thermal Cycle Dice Real Time System III (Takara Bio Inc., Shiga, Japan) with a PrimeTime® Gene Expression Master Mix (1,055,772; IDT) and a PrimeTime Std qPCR assay under the following conditions: incubation at 95 ◦C for 3 min, followed by 45 cycles of 95 ◦C for 5 s and 60 ◦C for 30 s. All the measurements were normalized to the housekeeping gene, beta-glucuronidase (Gusb). ..

Article Title: Inactivation of human norovirus by chlorous acid water, a novel chlorine-based disinfectant.
Article Snippet: After the neutralized samples were treated with 20 μg/ml of DNase-free ribonuclease (NIPPON GENE Co. Ltd.) at 25 ◦C for 30 min, viral RNA was extracted from 140 μl of the mixture using QIA viral RNA extraction kit (QIAGEN). .. HuNoV RNA copy number was measured by qPCR using Norovirus GI/GII kit (Takara Bio Co., Ltd.) and a Thermal Cycle Dice Real Time System III (TP970, Takara). ..

Gene Expression:

Incubation:

Amplification:

Article Title: The association between the adenoid microbiome and chronic otitis media with effusion in children differs according to age
Article Snippet: .. The DNA extracted from samples was amplified in a 25-μL reaction mixture containing 12.5 μL of TB Green Premix Ex Taq (Tli RNaseH Plus) (TaKaRa Bio, Inc.), 2 μM of each primer, and 1 μL of DNA template (a 10-fold dilution series of sample DNA) with the primer 340F (5′-TCC TAC GGG AGG CAG CAG-3′) and 518R (5′-ATT ACC GCG GCT GCT GG-3′) with a Thermal Cycle Dice Real-Time System III (TaKaRa Bio, Inc.). ..

Article Title: Disordered development of gut microbiome interferes with the establishment of the gut ecosystem during early childhood with atopic dermatitis
Article Snippet: .. The 16S rRNA gene was amplified with the primer 340 F (5′-TCC TAC GGG AGG CAG CAG-3′) and 518 R (5′-ATT ACC GCG GCT GCT GG-3′) using a Thermal Cycle Dice Real-Time System III (TaKaRa Bio, Inc.). .. Each sample was measured in triplicates in a 25-μL reaction containing 12.5 μL of TB Green Premix Ex Taq (Tli RNaseH Plus) (Cat #RR820B, TaKaRa Bio, Inc.), 2 μM of each primer, and 1 μL of DNA template (a 10-fold dilution series of sample DNA) with following amplification conditions: 95°C for 30s, followed by 40 cycles of denaturation at 95°C for 5 s and annealing at 60°C for 30s.

Article Title: Disordered development of gut microbiome interferes with the establishment of the gut ecosystem during early childhood with atopic dermatitis.
Article Snippet: .. The 16S rRNA gene was amplified with the primer 340 F (5′-TCC TAC GGG AGG CAG CAG-3′) and 518 R (5′-ATT ACC GCG GCT GCT GG-3′) using a Thermal Cycle Dice Real-Time System III (TaKaRa Bio, Inc.). .. Each sample was measured in triplicates in a 25-μL reaction containing 12.5 μL of TB Green Premix Ex Taq (Tli RNaseH Plus) (Cat #RR820B, TaKaRa Bio, Inc.), 2 μM of each primer, and 1 μL of DNA template (a 10-fold dilution series of sample DNA) with following amplification conditions: 95°C for 30s, followed by 40 cycles of denaturation at 95°C for 5 s and annealing at 60°C for 30s.

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